R-181. Ultrarapid technique for embryo cryopreservation with thawing in three steps
نویسندگان
چکیده
منابع مشابه
Survival of microorganisms after ultrarapid freezing and thawing.
Numerous reports during the past 75 years have described, usually in qualitative terms, the survival of various bacteria, yeasts, and fungi after exposure to very low temperatures (Smith, Biological Effects of Freezing and Supercooling, p. 74-137, The Williams & Wilkins Co., Baltimore, 1961). Although rates of cooling and warming have been shown to affect survival quantitatively (Mazur, Ann. N....
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Background: Couples receiving IVF treatment may choose to have embryos frozen, with the aim of creating a pregnancy and ultimately a live birth in future. Problems can arise when couples separate. Under UK law, embryos can only be transferred with the consent of both genetic parents. The issue came to public prominence in the case of Natallie Evans, who lost the opportunity to have her own gene...
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Investigation of the process of Peyer's patch (PP) formation has been hampered by difficulties in identifying its initial step in the embryo. In this study, we overcame this problem by means of whole-mount immunohistochemistry using mAb against the molecules which were expressed in the cells accumulated at the site of PP development. This method was sensitive enough to distinguish a minute cell...
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Cryoprotectants (CPAs) play a critical role in cryopreservation because they can resist the cell damage caused by the freezing process. Current state-of-the-art CPAs are mainly based on an organic solvent dimethyl sulfoxide (DMSO), and several DMSO-cryopreserved cell products have been brought to market. However, the intrinsic toxicity and complex freezing protocol of DMSO still remain as the b...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 1997
ISSN: 1460-2350,0268-1161
DOI: 10.1093/humrep/12.suppl_2.314